3ATG
endo-1,3-beta-glucanase from Cellulosimicrobium cellulans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-22 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 43 |
| Unit cell lengths | 71.309, 71.309, 60.069 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.654 - 1.660 |
| R-factor | 0.1466 |
| Rwork | 0.145 |
| R-free | 0.16640 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.307 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.720 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.054 | 0.130 |
| Number of reflections | 35478 | |
| <I/σ(I)> | 20.2 | 13.23 |
| Completeness [%] | 99.4 | 98.8 |
| Redundancy | 9.5 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 1.0M monoammonium dihydrogen phosphate, 0.1M trisodium citrate dehydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
