3ATA
Crystal Structure of the Kir3.2 Cytoplasmic Domain (Na+-free crystal soaked in 10 mM barium chloride and 10 mM Spermine)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-02 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.6000 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 81.691, 81.691, 172.429 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.990 - 3.490 |
R-factor | 0.2295 |
Rwork | 0.221 |
R-free | 0.28020 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.117 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.630 |
High resolution limit [Å] | 3.490 | 3.500 |
Rmerge | 0.163 | 0.564 |
Number of reflections | 104393 | |
<I/σ(I)> | 23.5 | 5.25 |
Completeness [%] | 100.0 | 99.7 |
Redundancy | 26.1 | 17.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 8 | 277 | Ethanol, pH 8.0, hanging drop, temperature 277K |