3ASY
ligand-free structure of uridine kinase from thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-31 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 70.364, 70.364, 179.243 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.800 - 2.400 |
Rwork | 0.233 |
R-free | 0.29950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | uridine cytidine kinase2 from Homo sapiens |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.640 | |
Number of reflections | 18465 | |
<I/σ(I)> | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 40% (v/v) isopropanol, 15%(w/v) PEG 8000, 0.1M imidazole, pH 6.5, VAPOR DIFFUSION, temperature 293K |