3ASG
MamA D159K mutant 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.570, 76.471, 104.587 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.720 - 2.330 |
R-factor | 0.2067 |
Rwork | 0.204 |
R-free | 0.25940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3as5 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.516 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.390 |
High resolution limit [Å] | 2.330 | 6.370 | 2.350 |
Rmerge | 0.123 | 0.047 | 0.483 |
Number of reflections | 15590 | ||
<I/σ(I)> | 8 | ||
Completeness [%] | 98.6 | 98.5 | 98.7 |
Redundancy | 5.2 | 5 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 286 | MgCl, PEG 3350, Tris, NaCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 286K |