3ASD
MamA R50E mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 43.649, 43.649, 207.449 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.710 - 2.450 |
| R-factor | 0.2745 |
| Rwork | 0.272 |
| R-free | 0.32500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3as5 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.851 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.450 | 6.650 | 2.450 |
| Rmerge | 0.048 | 0.031 | 0.285 |
| Number of reflections | 8008 | ||
| <I/σ(I)> | 23.4 | ||
| Completeness [%] | 98.0 | 80 | 100 |
| Redundancy | 10 | 7.1 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 286 | MES, NaCl, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 286K |
