3AS8
MamA MSR-1 P41212
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.888, 58.888, 144.092 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.940 - 2.000 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.22510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3as5 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.838 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 17.000 | 17.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.370 | 2.000 |
| Rmerge | 0.092 | 0.052 | 0.725 |
| Rmeas | 0.092 | ||
| Number of reflections | 17139 | ||
| <I/σ(I)> | 14.4 | ||
| Completeness [%] | 96.0 | 98.3 | 93.6 |
| Redundancy | 19 | 22.8 | 19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 286 | Ammonium sulfat, HEPES, NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 286K |
