3AS5
MamA AMB-1 P212121
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.750, 76.194, 105.057 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.830 - 2.000 |
| R-factor | 0.1791 |
| Rwork | 0.176 |
| R-free | 0.24100 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.666 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.410 | 2.000 |
| Rmerge | 0.063 | 0.045 | 0.259 |
| Number of reflections | 24938 | ||
| <I/σ(I)> | 17.9 | ||
| Completeness [%] | 99.7 | 99.1 | 99.8 |
| Redundancy | 6.7 | 6 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 286 | Tris, MgCl, PEG 3350, NaCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 286K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 286 | Tris, MgCl, PEG 3350, NaCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 286K |
