3ARK

Cl- binding hemoglobin component V form Propsilocerus akamusi under 1 M NaCl at pH 4.6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU ULTRAX 18
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-01-13
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	65.200, 75.250, 33.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.530 - 1.810
R-factor	0.19
Rwork	0.187
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1x3k
RMSD bond length	0.019
RMSD bond angle	1.700
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	CNX (2005)
Refinement software	CNX (2005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.280	1.870
High resolution limit [Å]	1.810	1.810
Rmerge	0.056	0.168
Number of reflections	15655
Completeness [%]	99.9	100
Redundancy	13.82	13.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	PEG3350, 1M sodium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K