3AR7
Calcium pump crystal structure with bound TNP-ATP and TG in the absence of Ca2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 71.515, 71.515, 586.334 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.150 |
R-factor | 0.2426 |
Rwork | 0.241 |
R-free | 0.26773 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1AGV |
RMSD bond length | 0.009 |
RMSD bond angle | 1.360 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 85138 | |
<I/σ(I)> | 2.271 | |
Completeness [%] | 99.7 | 98.6 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 6.1 | 283 | pH 6.1, MICRODIALYSIS, temperature 283K |