3AR4
Calcium pump crystal structure with bound ATP and TG in the absence of Ca2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 71.381, 71.381, 590.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.970 - 2.150 |
R-factor | 0.239 |
Rwork | 0.239 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1AGV |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.200 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.056 | 0.394 |
Number of reflections | 83710 | |
<I/σ(I)> | 21.9 | 3.13 |
Completeness [%] | 99.1 | 99.7 |
Redundancy | 7.8 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 6.1 | 283 | pH 6.1, MICRODIALYSIS, temperature 283K |
1 | MICRODIALYSIS | 6.1 | 283 | pH 6.1, MICRODIALYSIS, temperature 283K |