3AR3
Calcium pump crystal structure with bound ADP and TG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 71.309, 71.309, 590.026 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.960 - 2.300 |
R-factor | 0.239 |
Rwork | 0.239 |
R-free | 0.28300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1AGV |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.065 | 0.311 |
Number of reflections | 68737 | |
<I/σ(I)> | 12 | 3.2 |
Completeness [%] | 99.2 | 99.2 |
Redundancy | 8.2 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 6.1 | 283 | pH 6.1, MICRODIALYSIS, temperature 283K |