3AQP
Crystal structure of SecDF, a translocon-associated membrane protein, from Thermus thrmophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-15 |
Detector | RAYONIX MX225HE |
Spacegroup name | P 43 |
Unit cell lengths | 140.636, 140.636, 160.563 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.879 - 3.300 |
R-factor | 0.2991 |
Rwork | 0.298 |
R-free | 0.31900 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.298 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MLPHARE |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.300 |
Number of reflections | 41841 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 26% PEG 400, 0.2M SODIUM ACETETE, VAPOR DIFFUSION, HANGING DROP, temperature 293K |