3AQP
Crystal structure of SecDF, a translocon-associated membrane protein, from Thermus thrmophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-15 |
| Detector | RAYONIX MX225HE |
| Spacegroup name | P 43 |
| Unit cell lengths | 140.636, 140.636, 160.563 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.879 - 3.300 |
| R-factor | 0.2991 |
| Rwork | 0.298 |
| R-free | 0.31900 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.298 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.300 |
| Number of reflections | 41841 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 26% PEG 400, 0.2M SODIUM ACETETE, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
