3AQO

Structure and function of a membrane component SecDF that enhances protein export

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97942
Spacegroup name	C 1 2 1
Unit cell lengths	161.041, 35.829, 181.548
Unit cell angles	90.00, 113.68, 90.00

Refinement procedure

Resolution	36.860 - 2.600
R-factor	0.226
Rwork	0.223
R-free	0.28800
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.314
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.5.0066)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	24792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	18% PEG 3350, 0.6M Sodium thiocyanate, 3% trehalose, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP		293	18% PEG 3350, 0.6M Sodium thiocyanate, 3% trehalose, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP		293	18% PEG 3350, 0.6M Sodium thiocyanate, 3% trehalose, VAPOR DIFFUSION, SITTING DROP, temperature 293K