3AQG
Crystal structure of human pancreatic secretory protein ZG16b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.390, 66.631, 67.585 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.750 |
| R-factor | 0.223 |
| Rwork | 0.220 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3apa |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.496 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.164 | 0.501 |
| Number of reflections | 7619 | |
| <I/σ(I)> | 14 | 3.5 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 10.3 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.4M ammonium phosphate monobasic, 5%(v/v) 1,4-dioxane, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
