3AQE

Crystal structure of the extracellular domain of human RAMP2

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-06
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9788, 0.9792, 0.9640
Spacegroup name	P 21 21 21
Unit cell lengths	59.571, 89.729, 91.998
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.408 - 2.000
R-factor	0.2328
Rwork	0.230
R-free	0.27620
Structure solution method	MAD
RMSD bond length	0.003
RMSD bond angle	0.942
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	33866
<I/σ(I)>	19.3	7.7
Completeness [%]	100.0	99.1
Redundancy	6.62

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	0.1M Sodium-HEPES, 25% (v/v) PEG 400, 0.2M Calcium chloride di-hydrate, 0.15% Agarose , pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K