3AQE
Crystal structure of the extracellular domain of human RAMP2
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-06 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.9788, 0.9792, 0.9640 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.571, 89.729, 91.998 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.408 - 2.000 |
R-factor | 0.2328 |
Rwork | 0.230 |
R-free | 0.27620 |
Structure solution method | MAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.942 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 33866 | |
<I/σ(I)> | 19.3 | 7.7 |
Completeness [%] | 100.0 | 99.1 |
Redundancy | 6.62 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.1M Sodium-HEPES, 25% (v/v) PEG 400, 0.2M Calcium chloride di-hydrate, 0.15% Agarose , pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |