3AQC

M. luteus B-P 26 heterodimeric hexaprenyl diphosphate synthase in complex with magnesium and FPP analogue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2008-10-31
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	I 21 3
Unit cell lengths	189.803, 189.803, 189.803
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.610
R-factor	0.24039
Rwork	0.238
R-free	0.28152
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.003
RMSD bond angle	0.676
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC (5.5.0066)
Refinement software	REFMAC (5.5.0066)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	133.630	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	34831
<I/σ(I)>	27.5	9.1
Completeness [%]	100.0	100
Redundancy	15.9	13.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.08M Tris-HCl (pH 8.5), 0.16M Magnesium chloride hexahydrate, 24% (w/v) PEG 4000, 20% (v/v) Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 277K