3AP6
Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with lactose 3'-sulfate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2005-10-20 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.977980 |
Spacegroup name | P 1 |
Unit cell lengths | 41.376, 65.400, 71.693 |
Unit cell angles | 98.29, 105.43, 108.55 |
Refinement procedure
Resolution | 21.440 - 1.580 |
R-factor | 0.2018 |
Rwork | 0.200 |
R-free | 0.24179 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ap5 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.423 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.507 | 1.620 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.055 | 0.121 |
Number of reflections | 86082 | |
<I/σ(I)> | 5.8 | |
Completeness [%] | 94.4 | 91.5 |
Redundancy | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 0.275mM protein, 10mM Hepes-NaOH, 2.75mM lactose 3'-sulfate, 50mM sodium chloride, 0.5mM DTT, 50mM ammonium fluoride, 9% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |