3AP4
Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-03 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.92000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.528, 64.888, 71.584 |
| Unit cell angles | 98.41, 105.49, 108.66 |
Refinement procedure
| Resolution | 51.330 - 2.330 |
| R-factor | 0.19364 |
| Rwork | 0.191 |
| R-free | 0.25138 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.205 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.462 | 2.390 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.046 | 0.060 |
| Number of reflections | 26924 | |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 95.5 | 76.3 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 0.275mM protein, 10mM Hepes-NaOH, 2.75mM lactose, 50mM sodium chloride, 0.5mM DTT, 50mM ammonium fluoride, 12% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
