3AOS
Crystal structure of juvenile hormone binding protein from silkworm in complex with JH II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-10-22 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.044, 46.750, 72.169 |
| Unit cell angles | 90.00, 102.48, 90.00 |
Refinement procedure
| Resolution | 29.950 - 2.200 |
| R-factor | 0.24172 |
| Rwork | 0.239 |
| R-free | 0.29076 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a1z |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.630 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.075 | 0.312 |
| Number of reflections | 24811 | |
| <I/σ(I)> | 20.2 | 5.2 |
| Completeness [%] | 90.7 | 68.1 |
| Redundancy | 7 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 15% PEG 3350, 0.05M zinc acetate, 0.1M sodium citrate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
