3AOH
RNA polymerase-Gfh1 complex (Crystal type 1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2008-07-25 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9801 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 192.760, 260.700, 198.560 |
Unit cell angles | 90.00, 117.58, 90.00 |
Refinement procedure
Resolution | 49.870 - 4.100 |
R-factor | 0.262 |
Rwork | 0.262 |
R-free | 0.31800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 2O5I 3dxj and 2F23 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.700 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 4.100 |
Number of reflections | 131790 |
Completeness [%] | 96.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 50mM Hepes-NaOH buffer (pH 6.4), 5.5% PEG 8000, 300mM LiCl, 10mM MgCl2, 1% dimethyl sulfoxide, 5mM taurine, VAPOR DIFFUSION, SITTING DROP, temperature 293K |