3AO9
Crystal structure of the C-terminal domain of sequence-specific ribonuclease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-09 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 62.710, 62.710, 100.510 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.780 - 2.100 |
R-factor | 0.22359 |
Rwork | 0.222 |
R-free | 0.25866 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2djh |
RMSD bond length | 0.022 |
RMSD bond angle | 1.957 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 13723 | |
Completeness [%] | 98.6 | 99.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 278 | 50mM MES, 0.1M cadmium chloride, 25% PEG MME, 15% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K |