3AMH
crystal structure of cellulase 12A from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9732 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.679, 73.976, 181.031 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.090 |
| R-factor | 0.18 |
| Rwork | 0.177 |
| R-free | 0.22000 |
| Structure solution method | MIR |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.160 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.103 | 0.293 |
| Number of reflections | 33848 | |
| <I/σ(I)> | 20.2 | 6.8 |
| Completeness [%] | 98.7 | 99.6 |
| Redundancy | 5.4 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.1M Bis-Tris, 10% glycerol, 15% PEG3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
