3AMC
Crystal structures of Thermotoga maritima Cel5A, apo form and dimer/au
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.919, 78.340, 62.983 |
| Unit cell angles | 90.00, 97.25, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.400 |
| Rwork | 0.184 |
| R-free | 0.20860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.048 | 0.186 |
| Number of reflections | 118042 | |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.4M NaCl, 0.1M Tris, 32% PEG 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
