3AM2
Clostridium perfringens enterotoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2010-05-25 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 101.030, 101.030, 101.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.180 - 2.510 |
| R-factor | 0.21688 |
| Rwork | 0.214 |
| R-free | 0.26924 |
| Structure solution method | SAD |
| Starting model (for MR) | 2quo |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.637 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.087 | 0.478 |
| Number of reflections | 12081 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 20.7 | 22.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 20% PEG 4000, 0.1M Tris-HCl, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
