3AM1
Crystal structure of O-Phosphoseryl-tRNA kinase complexed with anticodon-stem/loop truncated tRNA(Sec)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-19 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.000 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 46.749, 46.749, 459.712 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.463 - 2.400 |
R-factor | 0.2324 |
Rwork | 0.229 |
R-free | 0.29190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3a4n |
RMSD bond length | 0.006 |
RMSD bond angle | 1.536 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.6_289) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
Rmerge | 0.111 | 0.054 | 0.392 |
Number of reflections | 22946 | ||
<I/σ(I)> | 25.886 | ||
Completeness [%] | 97.6 | 97.6 | 79.4 |
Redundancy | 14.3 | 19.3 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 100mM phosphate buffer (pH 6.2), 0.2M NaCl, 41% PEG 200, vapor diffusion, sitting drop, temperature 293K, VAPOR DIFFUSION, SITTING DROP |