3ALY
Crystal Structure of RNase HI from Sulfolobus tokodaii with C-terminal deletion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-04 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 40.975, 41.018, 43.534 |
Unit cell angles | 89.72, 89.71, 86.76 |
Refinement procedure
Resolution | 20.480 - 1.660 |
R-factor | 0.19996 |
Rwork | 0.197 |
R-free | 0.25048 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ehg |
RMSD bond length | 0.011 |
RMSD bond angle | 1.391 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.720 |
High resolution limit [Å] | 1.660 | 1.660 |
Rmerge | 0.034 | 0.218 |
Number of reflections | 32375 | |
<I/σ(I)> | 37.21 | 6.14 |
Completeness [%] | 96.2 | 94.3 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 20% PEG 3000, 0.1M citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |