3ALY
Crystal Structure of RNase HI from Sulfolobus tokodaii with C-terminal deletion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.975, 41.018, 43.534 |
| Unit cell angles | 89.72, 89.71, 86.76 |
Refinement procedure
| Resolution | 20.480 - 1.660 |
| R-factor | 0.19996 |
| Rwork | 0.197 |
| R-free | 0.25048 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ehg |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.391 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.720 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.034 | 0.218 |
| Number of reflections | 32375 | |
| <I/σ(I)> | 37.21 | 6.14 |
| Completeness [%] | 96.2 | 94.3 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 20% PEG 3000, 0.1M citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
