3ALX
Crystal structure of the measles virus hemagglutinin bound to its cellular receptor SLAM (MV-H(L482R)-SLAM(N102H/R108Y) fusion)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 187.713, 170.074, 110.681 |
| Unit cell angles | 90.00, 117.28, 90.00 |
Refinement procedure
| Resolution | 19.970 - 3.150 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zb6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.138 | 0.496 |
| Number of reflections | 52457 | |
| <I/σ(I)> | 10.3 | 2.9 |
| Completeness [%] | 99.5 | 98.8 |
| Redundancy | 4.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | vapor diffusion, floating drop | 8.3 | 293 | 0.1M imidazole pH8.3, 0.5M (NH4)2SO4, 0.7M Li2SO4, 0.05M Li(AcO)2, 1% ethylene glycol, vapor diffusion, floating drop, temperature 293K |
