3ALW
Crystal structure of the measles virus hemagglutinin bound to its cellular receptor SLAM (Form I, MV-H-SLAM(N102H/R108Y) fusion)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 209.912, 209.912, 180.538 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.890 - 3.550 |
| R-factor | 0.25 |
| Rwork | 0.250 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zb6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.680 |
| High resolution limit [Å] | 3.550 | 3.550 |
| Rmerge | 0.074 | 0.498 |
| Number of reflections | 28716 | |
| <I/σ(I)> | 38.6 | 6.5 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 20 | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, FLOATING DROP | 5.8 | 293 | 0.1M imidazole pH5.8, 0.5M (NH4)2SO4, 0.7M Li2SO4, 3% ethylene glycol, VAPOR DIFFUSION, FLOATING DROP, temperature 293K |
