3AL9
Mouse Plexin A2 extracellular domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-12 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.702, 60.677, 95.385 |
| Unit cell angles | 109.80, 92.47, 112.53 |
Refinement procedure
| Resolution | 42.560 - 2.100 |
| R-factor | 0.20777 |
| Rwork | 0.205 |
| R-free | 0.25748 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1shy |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.272 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.560 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.069 | 0.289 |
| Number of reflections | 60889 | |
| <I/σ(I)> | 15.4 | 4 |
| Completeness [%] | 98.0 | 97 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 24-28%(wt/vol) PEG 3350, 0-0.2M NaCl, 0.1M Tris-Cl pH 8.0-8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
