3AKS
Crystal structure of xylanase from Trichoderma longibrachiatum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-02 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.8000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.629, 38.915, 40.199 |
| Unit cell angles | 90.00, 110.47, 90.00 |
Refinement procedure
| Resolution | 21.920 - 0.970 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.19200 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.000 |
| High resolution limit [Å] | 0.970 | 0.970 |
| Rmerge | 0.055 | 0.359 |
| Number of reflections | 94660 | |
| <I/σ(I)> | 9.9 | 3.9 |
| Completeness [%] | 97.6 | 92.8 |
| Redundancy | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | oil microbatch | 8.7 | 293 | 4M sodium formate, pH 8.7, oil microbatch, temperature 293K |
