3AKR
Crystal structure of xylanase from Trichoderma longibrachiatum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-02 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.8000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.755, 38.904, 40.214 |
| Unit cell angles | 90.00, 110.48, 90.00 |
Refinement procedure
| Resolution | 27.020 - 1.100 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1enx |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.140 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.077 | 0.444 |
| Number of reflections | 66824 | |
| <I/σ(I)> | 7 | 3.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | oil microbatch | 8.7 | 293 | 4M sodium formate, pH 8.7, oil microbatch, temperature 293K |
