3AKR
Crystal structure of xylanase from Trichoderma longibrachiatum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-03-02 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 0.8000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.755, 38.904, 40.214 |
Unit cell angles | 90.00, 110.48, 90.00 |
Refinement procedure
Resolution | 27.020 - 1.100 |
R-factor | 0.182 |
Rwork | 0.182 |
R-free | 0.18400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1enx |
RMSD bond length | 0.012 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.140 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.077 | 0.444 |
Number of reflections | 66824 | |
<I/σ(I)> | 7 | 3.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | oil microbatch | 8.7 | 293 | 4M sodium formate, pH 8.7, oil microbatch, temperature 293K |