3AKQ
Crystal structure of xylanase from Trichoderma longibrachiatum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-11-19 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.921, 37.516, 84.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.140 - 0.970 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1enx |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.000 |
| High resolution limit [Å] | 0.970 | 0.970 |
| Rmerge | 0.041 | 0.470 |
| Number of reflections | 116654 | |
| <I/σ(I)> | 12.7 | 3.5 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 6.7 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | oil microbatch | 6.5 | 293 | 18% PEG 8000, 0.2M zinc acetate, pH 6.5, oil microbatch, temperature 293K |
