3AKJ
Crystal structure of A Helicobacter pylori proinflammatory kinase CtkA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 255.195, 56.980, 48.638 |
Unit cell angles | 90.00, 93.28, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.1962 |
Rwork | 0.194 |
R-free | 0.22970 |
Structure solution method | MAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 47319 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 295 | 100mM Tris-HCl, 1.5M AMS, 15% glycerol, pH 8.5, VAPOR DIFFUSION, temperature 295K |
1 | VAPOR DIFFUSION | 8.5 | 295 | 100mM Tris-HCl, 1.5M AMS, 15% glycerol, pH 8.5, VAPOR DIFFUSION, temperature 295K |