3AK5
Hemoglobin protease (Hbp) passenger missing domain-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 292.803, 53.936, 335.953 |
| Unit cell angles | 90.00, 107.32, 90.00 |
Refinement procedure
| Resolution | 48.560 - 2.200 |
| R-factor | 0.2255 |
| Rwork | 0.226 |
| R-free | 0.28010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wxr |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.949 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.213 | |
| Number of reflections | 239062 | |
| Completeness [%] | 93.0 | 71.9 |
| Redundancy | 3.69 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 9% PEG 1000, 10% PEG 8000, 0.1M NaOAc pH 5.0, 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
