3AK2
Superoxide dismutase from Aeropyrum pernix K1, Mn-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-16 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.064, 71.782, 76.853 |
| Unit cell angles | 90.00, 91.81, 90.00 |
Refinement procedure
| Resolution | 31.860 - 1.350 |
| R-factor | 0.1879 |
| Rwork | 0.187 |
| R-free | 0.20281 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3ak1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.052 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.048 | 0.361 |
| Number of reflections | 161154 | |
| <I/σ(I)> | 15.9 | 5.8 |
| Completeness [%] | 98.0 | 97 |
| Redundancy | 7.3 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM HEPES, 8% PEG 6000, 8% ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
