3AK0
Crystal Structure of Ancestral Congerin Con-anc'-N28K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-14 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.828, 55.354, 59.453 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.110 - 1.590 |
| R-factor | 0.18164 |
| Rwork | 0.180 |
| R-free | 0.21176 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1is3 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.523 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.590 | 1.600 |
| Number of reflections | 36540 | |
| <I/σ(I)> | 34 | 6.7 |
| Completeness [%] | 99.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.1M DL-malic acid, 1mM lactose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
