3AJZ
Crystal Structure of Ancestral Congerin Con-anc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-26 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 53.908, 77.519, 88.374 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.270 - 1.500 |
| R-factor | 0.20448 |
| Rwork | 0.204 |
| R-free | 0.21714 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1is3 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.521 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 29601 | |
| <I/σ(I)> | 42.2 | 2.8 |
| Completeness [%] | 99.0 | 92.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.1M DL-malic acid, 1mM lactose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
