3AJW
Structure of FliJ, a soluble component of flagellar type III export apparatus
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 35 |
Detector technology | CCD |
Collection date | 2007-12-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0082 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 52.925, 52.925, 192.597 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.300 - 2.100 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.25500 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.000 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.520 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.080 | 0.317 |
Number of reflections | 10032 | |
<I/σ(I)> | 25 | 7 |
Completeness [%] | 99.7 | 100 |
Redundancy | 10.9 | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.8 | 293 | 27% PEG 300, 0.1M Phospho-Citrate, 0.35M NaCl, pH 3.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |