3AJC
Structure of the MC domain of FliG (PEV), a CW-biased mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 35 |
| Detector technology | CCD |
| Collection date | 2007-12-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 62 |
| Unit cell lengths | 93.150, 93.150, 48.390 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.560 - 2.300 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.490 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.065 | 0.336 |
| Number of reflections | 10581 | |
| <I/σ(I)> | 21.3 | 5.3 |
| Completeness [%] | 97.9 | 97.9 |
| Redundancy | 6.2 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 277 | 50% PEG 200, 0.1M Phospho-Citrate, 0.2M NaCl, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
