3AJ7
Crystal Structure of isomaltase from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.516, 115.538, 61.756 |
| Unit cell angles | 90.00, 91.05, 90.00 |
Refinement procedure
| Resolution | 18.700 - 1.300 |
| R-factor | 0.17311 |
| Rwork | 0.172 |
| R-free | 0.18607 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a47 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.397 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.038 | 0.144 |
| Number of reflections | 155721 | |
| <I/σ(I)> | 34 | 8.4 |
| Completeness [%] | 95.1 | 93.1 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 288 | 19% PEG 3350, 0.05N HEPES, 0.2M lithium acetete, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
