3AJ3
Crystal structure of selenomethionine substituted 4-pyridoxolactonase from Mesorhizobium loti
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.777, 39.214, 84.191 |
| Unit cell angles | 90.00, 117.92, 90.00 |
Refinement procedure
| Resolution | 28.989 - 1.577 |
| R-factor | 0.164 |
| Rwork | 0.163 |
| R-free | 0.19000 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.095 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | RESOLVE |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.990 | 1.640 |
| High resolution limit [Å] | 1.577 | 1.577 |
| Rmerge | 0.051 | 0.284 |
| Number of reflections | 32428 | |
| <I/σ(I)> | 22.6 | 7.9 |
| Completeness [%] | 97.8 | 93 |
| Redundancy | 6.7 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 30% PEG4000, 0.15M ammonium acetate, 0.1M sodium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
