3AIH
Human OS-9 MRH domain complexed with alpha3,alpha6-Man5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 73.100, 73.100, 180.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.100 |
R-factor | 0.23 |
Rwork | 0.228 |
R-free | 0.27700 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.663 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.098 | 0.469 |
Number of reflections | 17480 | |
<I/σ(I)> | 28.5 | 5.5 |
Completeness [%] | 99.7 | 100 |
Redundancy | 11.4 | 11.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 303 | 1.9M ammonium sulfate, 0.1M Tris-HCl, 2% PEG400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |