3AHY
Crystal structure of beta-glucosidase 2 from fungus Trichoderma reesei in complex with Tris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 92.830, 103.511, 94.802 |
Unit cell angles | 90.00, 105.37, 90.00 |
Refinement procedure
Resolution | 29.900 - 1.630 |
R-factor | 0.16704 |
Rwork | 0.164 |
R-free | 0.21771 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2e3z |
RMSD bond length | 0.005 |
RMSD bond angle | 1.439 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.690 |
High resolution limit [Å] | 1.630 | 1.630 |
Rmerge | 0.088 | 0.503 |
Number of reflections | 210766 | |
<I/σ(I)> | 20.9 | 4.5 |
Completeness [%] | 96.9 | 95.3 |
Redundancy | 5.6 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1M Tris, 20-22%(w/v) PEG 3350, 0.23M MgCl2, 12.5mM sodium formate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |