3AHR
Inactive human Ero1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-04 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.90 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 54.698, 139.094, 143.491 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.010 - 3.070 |
| R-factor | 0.22779 |
| Rwork | 0.224 |
| R-free | 0.30253 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rp4 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.623 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.010 | 3.240 |
| High resolution limit [Å] | 3.070 | 3.070 |
| Rmerge | 0.063 | 0.428 |
| Number of reflections | 10434 | |
| <I/σ(I)> | 13.8 | 2.9 |
| Completeness [%] | 98.7 | 93.1 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 20% PEG1500, 80mM imidazole pH8.0, VAPOR DIFFUSION, temperature 293K |
