3AH7
Crystal structure of the ISC-like [2Fe-2S] ferredoxin (FdxB) from Pseudomonas putida JCM 20004
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 87.575, 87.575, 73.139 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.920 - 1.900 |
R-factor | 0.18341 |
Rwork | 0.182 |
R-free | 0.21562 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i7h |
RMSD bond length | 0.013 |
RMSD bond angle | 1.558 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.810 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.116 | 0.350 |
Number of reflections | 13390 | |
<I/σ(I)> | 8.7 | |
Completeness [%] | 98.6 | 97.8 |
Redundancy | 9.1 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.6M trisodium citrate dihydrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |