3AGY
Crystal structure of human Hsp40 Hdj1 peptide-binding domain complexed with a C-terminal peptide of Hsp70
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-06-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9780 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.746, 41.132, 62.865 |
| Unit cell angles | 90.00, 97.42, 90.00 |
Refinement procedure
| Resolution | 42.710 - 1.850 |
| R-factor | 0.226 |
| Rwork | 0.225 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.435 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.700 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.056 | 0.482 |
| Number of reflections | 45431 | |
| <I/σ(I)> | 23.1 | 1.5 |
| Completeness [%] | 99.1 | 96.9 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 18% PEG 3350, 0.1M Na citrate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
