3AGX
Crystal structure of human Hsp40 Hdj1 peptide-binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-05-19 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.9780 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.252, 40.921, 62.650 |
| Unit cell angles | 90.00, 96.62, 90.00 |
Refinement procedure
| Resolution | 42.030 - 1.850 |
| R-factor | 0.229 |
| Rwork | 0.228 |
| R-free | 0.24700 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.532 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.600 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.101 | 0.693 |
| Number of reflections | 42368 | |
| <I/σ(I)> | 1.3 | |
| Completeness [%] | 94.3 | 64.8 |
| Redundancy | 4.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 13% PEG 3350, 0.1M Na citrate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
