3AGW
Crystal Structure of the Cytoplasmic Domain of G-Protein-Gated Inward Rectifier Potassium Channel Kir3.2 in the absence of Na+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 81.943, 81.943, 172.769 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.730 - 2.200 |
| R-factor | 0.19978 |
| Rwork | 0.198 |
| R-free | 0.23577 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e4f |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.270 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.730 | |
| High resolution limit [Å] | 2.199 | 2.200 |
| Rmerge | 0.063 | 0.063 |
| Number of reflections | 15254 | |
| <I/σ(I)> | 29 | 29 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 9.5 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 7.5-12.5% EtOH, 0.1M Imidazole-HCl, 0.1M MgCl2, 0.003M spermine, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
