3AGK
Crystal structure of archaeal translation termination factor, aRF1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-09 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.443, 80.430, 87.873 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.558 - 2.100 |
R-factor | 0.2125 |
Rwork | 0.210 |
R-free | 0.26000 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.116 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 27344 | |
<I/σ(I)> | 40.6 | 2.5 |
Completeness [%] | 98.3 | 95.9 |
Redundancy | 5.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 200mM Ammonium citrate tribasic, 12% PEG MME 2000, 10mM ATP, pH 7.0, VAPOR DIFFUSION, temperature 293K |