3AGI
High resolution X-ray analysis of Arg-lysozyme complex in the presence of 500 mM Arg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.033, 78.033, 37.752 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.020 - 1.200 |
| R-factor | 0.13866 |
| Rwork | 0.137 |
| R-free | 0.16664 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a34 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.790 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.052 | 0.460 |
| Number of reflections | 34994 | |
| <I/σ(I)> | 4.3 | |
| Completeness [%] | 100 | |
| Redundancy | 14.7 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 293 | 0.05 M Sodium Acetate 1.6 M Sodium Chloride 0.5 M Arg, pH 4.5, VAPOR DIFFUSION, temperature 293K |
